FastQCFastQC Report
Sun 14 Apr 2019
SRR6576278_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576278_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222787
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5590.25091230637335216No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3190.14318609254579487No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2920.13106689349019468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.12478286435025382No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2750.12343628667740936No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2740.12298742745312788No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2620.11760111676175002No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2620.11760111676175002No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2330.10458419925758683No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2310.10368648080902386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACTA450.001009804141.797849
ATGGGAG3600.039.159095
TACATGG17800.038.5517582
GTACATG18050.038.3384481
ACATGGG18000.037.0706063
CATGGGA9900.035.1245164
ATAGTGT1001.399143E-632.9158028
ATGGGTA2000.032.8936355
GGTACCT1755.456968E-1232.244058
GGGTACC1755.456968E-1232.2223367
GTATCAA7800.031.3836291
CATAGGA753.2708433E-431.3272724
TAGTACT600.004157784431.327274
CATGGGT3550.030.4448154
ACATAGG650.00614947528.9174843
CATGGGG6000.028.1945464
ATGGGAT2900.027.5463945
ACGGCAA350.008809661526.87004392-93
CGAAAAT350.00883865426.85194860-61
TGGGAGA2357.2759576E-1225.994976