FastQCFastQC Report
Sun 14 Apr 2019
SRR6576279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306009
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7570.2473783450813538No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4000.13071510968631642No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3890.12712044416994273No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3880.12679365639572693No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3690.1205846886856269No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3620.11829717426611636No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3420.11176141878180054No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3310.10816675326542682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.10097742223267943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG200.002161697770.498046
ACATGGG20700.039.7331923
GTACATG22100.038.0854841
TACATGG22300.037.743912
ATGGGAG3950.035.695215
CATGGGA10700.033.821494
GTATCAA10150.033.8185921
CATGGGC3500.033.5704964
CATGGGT4700.030.999144
ATGGGTA3150.029.840445
CATATAG650.006148045428.9222743
CATGGGG7600.028.4465774
TCAACGC11700.028.1188764
ATCAACG11750.027.9992243
GGGTACC2900.027.5509597
AACGCAG12050.027.3021456
CAACGCA12100.027.1893275
TAATACC909.503216E-426.1103844
ATGGGAT2750.025.6356495
GGTACCT3150.025.3643748