FastQCFastQC Report
Sun 14 Apr 2019
SRR6576280_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576280_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303719
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7760.25549932668025377No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4630.15244354156308956No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4450.14651701078957852No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3870.1274204116304874No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3790.12478639795337138No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.12379864282445288No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3630.11951837059913935No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3390.11161632956779129No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3280.10799456076175674No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3230.10634830221355925No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3090.10173877827860621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC156.901365E-493.9863
TTATCGC250.0052217956.42876494
TTAACAC450.001013518541.7715573
CATGGGG7200.036.5501144
ATGGGTA3300.035.6007585
GTATCAA9550.035.522851
GGGTACC2650.035.466427
ACATGGG20500.035.302063
CATGGGT4400.034.1767274
GTACATG22100.034.1119421
TACATGG21650.034.083682
GGTACCT2800.033.5885478
ATAGACG600.004145844431.3493148
TAGACGA650.00613183828.9378289
AACGCAG11250.028.8223766
TCAACGC11500.027.7871674
CAACGCA11800.027.478965
ATGGGGA4450.027.4565875
CTAATCG350.00881086326.87083892-93
TATACAG1057.4114236E-526.8531445