FastQCFastQC Report
Sun 14 Apr 2019
SRR6576281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250679
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7770.3099581536546739No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4320.17233194643348665No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4050.16156119978139372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.14640237115992963No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3540.1412164561052182No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3510.1400197064772079No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3390.13523270796516662No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3370.1344348748798264No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3170.12645654402642423No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3000.11967496280103239No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2590.10331938455155797No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2590.10331938455155797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT551.27929525E-859.8271984
ATGGGTA2750.039.3150145
GGTACCT2450.038.3654758
ATGGGAC757.4126838E-637.6056675
CTGTGCG1003.3105607E-837.5981649
TTCAAGA651.409089E-436.1592942
GGGTACC2600.036.152087
CATGGGT3550.035.7518654
CTACACT801.1538506E-535.2553144
GTCCTAC550.002700375434.2142751
ATAGGTG550.002710970834.186975
GGCGTCG550.002713624934.180158
GCGTCGG550.002713624934.180159
GTATCAA8800.033.6796761
TGGGTAT702.1807756E-433.569796
GTCGTCA352.183878E-433.5697924-25
CACCGTC702.1807756E-433.569797
GTACATG15150.032.2946621
ACATGGG14850.031.6546023
CCGAACT753.250147E-431.3630871
Produced by FastQC (version 0.11.2)