FastQCFastQC Report
Sun 14 Apr 2019
SRR6576281_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576281_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250679
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6980.2784437467837354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.19746368862170344No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4060.16196011632406385No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3520.140418623019878No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3490.1392218733918677No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3460.13802512376385737No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3400.13563162450783672No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3200.12765329365443454No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3060.12206846205705305No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2990.11927604625836229No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2970.11847821317302208No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2900.11568579737433132No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2710.10810638306359927No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2660.10611180035024871No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2630.1049150507222384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCA405.6700164E-446.9969069
AAGCTAA405.6700164E-446.9969069
GTACTAG801.13206515E-535.3534661
CTAGGCA959.4163806E-734.62934
GTATCAA7800.034.4469681
AATGCGA352.1815374E-433.57591620-21
CCAATCC702.1809927E-433.5692183
GTACATG14850.032.0601581
TCGAATT300.004156043331.33752320-21
ACATGGG14850.031.3312723
CGCCGGT1501.0622898E-931.3312727
TCTATTC1052.0579173E-631.3312728
TACATGG15600.030.4396322
CATGGGG6650.030.388984
CGCGGAA404.778922E-429.37306882-83
CAATGCG404.778922E-429.37306818-19
GTACTTG1452.568413E-829.2580411
GTGTTAT650.006057719729.0079731
TGCTAGT650.006135116328.9327162
GGTATGA650.006147092728.9211738
Produced by FastQC (version 0.11.2)