FastQCFastQC Report
Sun 14 Apr 2019
SRR6576284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355915
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7770.21831055167666436No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4590.12896337608698707No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3990.11210541842855738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.10480030344323785No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3580.10058581402863044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10600.039.9377751
TACATGG22100.034.9058952
GTACATG22450.033.942661
CATGGGT5150.033.765684
ACATGGG22550.033.555243
CATGGGG7700.032.959764
ATCAACG13250.031.5685843
AACGCAG13450.031.4485936
TCAACGC13450.031.0991634
CAACGCA13550.030.869655
CTTATGC804.775416E-429.373863
AACAGCG650.006149707428.9219537
ATGGGTA3450.028.6075845
TCTAGAC1154.186966E-628.6075843
TATCAAC15050.028.1289312
GTATATA2850.028.0576481
ACGCAGA15700.026.941637
ATGGGTG1755.527909E-926.85615
AACGTAC350.00883547726.85609874-75
GAGTCGC350.00883547726.85609872-73
Produced by FastQC (version 0.11.2)