FastQCFastQC Report
Sun 14 Apr 2019
SRR6576284_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576284_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355915
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6710.1885281598134386No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4100.11519604399926948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.11294831631147888No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3730.10480030344323785No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3720.10451933748226402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11150.038.053591
GTATAGG651.3863211E-436.2647441
GTACATG22500.032.896151
TACATGG22450.032.8675162
ACATGGG22250.032.3180353
TCAACGC13550.031.5635384
AACGCAG14250.031.002496
ATCAACG13550.030.5229823
CAACGCA13950.030.3215855
GTATTAC804.689454E-429.4651031
CATGGGG7550.028.0123254
ACGCAGA16300.026.815077
ATGGGAC2305.456968E-1226.5643445
TATCAAC15900.026.307312
CGCAGAG16500.026.20528
CATGGGT4700.025.9991464
ACCGTCG1101.01663E-425.6355198
ATACCGT1101.01663E-425.6355196
CATGGGA13350.025.347484
GGGTACC2601.8189894E-1225.3068587
Produced by FastQC (version 0.11.2)