FastQCFastQC Report
Sun 14 Apr 2019
SRR6576285_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576285_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319200
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6880.2155388471177945No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4230.1325187969924812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4080.12781954887218044No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3630.1137218045112782No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3590.112468671679198No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3530.11058897243107771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG21650.038.1127971
TACATGG21350.037.6407052
ACATGGG22050.035.5932273
GTATCAA11550.035.516321
ATGGGTA2650.035.4685635
AGACCGT801.1567321E-535.2468876
GGGTACC2600.032.5355877
CATGGGG6850.031.5592564
GGTACCT2750.030.7850348
CATGGGT4900.030.6911684
TGGGTAC3100.030.3199026
AACGCAG13750.028.3684046
CATGGGA11600.028.3595644
GCCATAC856.678783E-427.7358671
CAACGCA14350.027.1822685
ATAGTGC700.00883043726.854776
ATCAACG14450.026.6689243
TCAACGC14650.026.6256334
ATGGGAG4700.024.9977915
ACGCAGA15700.024.844947
Produced by FastQC (version 0.11.2)