FastQCFastQC Report
Sun 14 Apr 2019
SRR6576286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276855
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5960.21527514402846257No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3370.12172436835166424No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3270.11811236929078399No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.11738996947860793No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3230.1166675696664319No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3220.11630636976034385No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3180.11486157013599176No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3110.11233317079337558No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3100.11197197088728757No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2940.10619277238987919No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2790.1007747737985588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG20650.038.9331131
GTATCAA8950.038.8733331
TACATGG20950.038.6000182
ACATGGG20250.038.2952653
ATGGGAG5000.035.719045
GGGTACC2400.035.2490547
GGTATAT550.002709017134.193251
GGTACCT2500.033.839098
CATGGGT4300.031.6968214
ATCCGCG300.004159683431.33249392-93
ATCAACG11100.031.332493
AACGCAG11300.031.1938516
CATGGGG6500.031.091474
ATGGGTA3050.030.8188445
CAACGCA11600.029.981955
TCAACGC11600.029.981954
ATGGGCG1102.9583498E-629.9082875
CATGGGA11650.029.0464294
GTTGTAT650.006136175228.932751
CATGGGC4450.028.5160874