FastQCFastQC Report
Sun 14 Apr 2019
SRR6576286_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576286_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276855
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5080.1834895522927164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.14267396290476966No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3830.13833956403171335No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3230.1166675696664319No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3000.10835997182640732No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2990.1079987719203193No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2990.1079987719203193No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2910.10510917267161511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11100.039.481931
GTACATG20400.037.8836751
ACATGGG20050.037.7373853
CATGGGG7100.037.7291344
GTACTAG500.001678002937.6988751
TGTACGA250.001700478737.60354274-75
TACATGG20500.037.596752
AACGCAG12800.033.0440186
ATCAACG13300.032.508473
CAACGCA13550.031.908685
TCAACGC13600.031.791374
ACCGAGG600.00415733631.3306248
CATGGGA11300.030.7761024
GTACTAA650.006067828728.9991381
TATCAAC15100.028.9445172
CTAAAGC650.006148807228.9205783
CGCAGAG14550.028.4236588
GTATTAT1005.214926E-528.2741581
ACGCAGA14800.028.2610687
ATGGGGA4100.027.5098175