FastQCFastQC Report
Sun 14 Apr 2019
SRR6576287_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576287_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256637
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5490.21392082981019886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.14923802881112233No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3560.13871733226307975No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3250.12663801400421607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3140.122351804299458No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2910.11338972946223654No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2910.11338972946223654No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2810.10949317518518374No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2800.10910351975747846No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2770.10793455347436261No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2690.10481731005272038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGA450.001003637141.852311
GTATCAA10400.038.0292661
TTAGGGC757.429602E-637.593694
GTATACT757.429602E-637.593694
ACATGGG19200.035.488833
GTACATG19700.035.372641
TACATGG19900.034.9489062
GTATAGA702.1575764E-433.6313251
CATGGGT3800.033.389134
AACGCAG12000.033.286086
ATGGGAG3300.032.752085
CAACGCA12300.032.092175
TCAACGC12350.031.9622444
ATCAACG12400.031.8333633
CATGGGG5850.031.3280744
ATGGGTA2400.031.3280725
CGACAAT300.00416227531.32807286-87
ACGCAGA13850.028.839927
ATGGGGA3650.028.3240095
CGCAGAG14150.028.2504948