FastQCFastQC Report
Sun 14 Apr 2019
SRR6576288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360405
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6010.16675684299607385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA250.00523374356.3983359
CCGCTAA250.00523374356.3983354
GTATCAA8400.040.8440321
CATGGGT4650.039.418194
TACATGG20650.035.5050052
GTACATG20700.035.1921961
ACATGGG19950.034.866143
GCGCAAA1201.647386E-731.33240994
ATCAACG11200.031.0526563
ATGGGTA3200.030.8428385
TCAACGC11300.030.3619374
AACGCAG11500.029.83396
CAACGCA11550.029.7047525
TGGGTAC3450.028.6078516
GGGTACC3500.028.199177
GGTACCT3750.027.572528
GTTCTAC1205.829941E-627.4158571
CATGGGA8850.027.0839484
TTTCGCG350.00883514626.8563524-25
CGCAGAG13000.026.7530568
Produced by FastQC (version 0.11.2)