FastQCFastQC Report
Sun 14 Apr 2019
SRR6576288_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576288_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360405
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5310.14733424896990885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3950.1095989234333597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8450.048.4963261
TACTAGC450.001011836841.787772
CTAATAG704.6187834E-640.2953453
ATCAACG10450.039.1385423
CAACGCA10750.038.483625
TCAACGC10800.037.8701634
GTACATG18800.037.8325271
ACATGGG18550.037.761053
TACATGG18700.037.7095532
AACGCAG11150.037.5246666
TATCAAC11900.034.3695642
ACGCAGA12500.033.4627157
CGCAGAG13100.031.9300758
CATGGGA10200.030.879934
ATGGGTA2600.030.738125
ATGGGGG3200.029.3820255
CATGGGG7450.028.3960534
TTCGGAC350.0088355326.85611246-47
GCAGAGT15950.026.2246999
CTACGAC909.4917E-426.1173554
Produced by FastQC (version 0.11.2)