FastQCFastQC Report
Sun 14 Apr 2019
SRR6576290_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576290_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237172
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7350.30990167473394836No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4250.179194845934596No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3850.1623294486701634No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3410.1437775116792876No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3260.13745298770512537No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3260.13745298770512537No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3180.1340799082522389No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2950.12438230482519017No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2880.12143086030391445No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2690.11341979660330899No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2570.10836017742397923No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2540.10709527262914677No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2510.10583036783431433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.10372219317626027No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2420.10203565344981702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC250.00523343956.393172
CTTATAG250.00523343956.393173
CATGGGT3500.036.252754
GTACATG15550.036.031861
TACATGG16000.034.952022
ACATGGG15950.034.177683
TCATAGG550.002714293834.177683
GTATCAA7800.033.8036461
GTATAAG902.5540756E-531.38911
TAGACCC600.004156850731.3295384
GATTGGC600.004156850731.3295389
CTGATAC600.004156850731.3295383
AACGCAG8850.029.7365136
GTACAGG804.7201532E-429.4272821
GGTACCT2450.028.7720268
ATGGGTA2300.028.605235
ATCAACG9300.028.297653
CAACGCA9750.027.4735955
CATGGGG7200.027.4133474
TCAACGC9700.027.1307354
Produced by FastQC (version 0.11.2)