FastQCFastQC Report
Sun 14 Apr 2019
SRR6576291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220061
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7170.32581875025561097No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3940.17904126583083782No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3710.16858961833309855No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3690.16768077942025167No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3440.1563202930096655No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3240.14723190388119659No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2850.1295095450806822No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2820.12814628671141182No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2760.12541976997287116No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2710.12314767269075394No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2700.12269325323433049No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2630.11951231703936635No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2610.11860347812651946No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2460.11178718628016776No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2460.11178718628016776No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2390.10860625008520365No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2330.10587973334666298No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2320.10542531389023953No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC2240.10178995823885197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA156.9024257E-493.973644
CGCTAAA200.00216305470.480235
CCCGCTA250.00523623956.3841863
ATAACAC500.001701380437.589463
GTACATG17850.033.708951
ACATGGG17750.033.618743
TACATGG18150.033.1517752
GTATATA902.5814004E-531.338791
ATATAAC600.00415944731.3245473
CATGGGT3650.030.8954454
ATGGGTA2600.030.7221535
CATGGGC2150.030.596074
CATGGGG6850.030.1813164
TGTATAC650.0061519328.9149683
TAGGATG650.0061519328.9149685
ATGGGAG2950.028.6699245
GTATACT700.00883285826.8496114
CTTAGCT1606.881601E-826.4300884
TATATGT909.508711E-426.1037884
CATGGGA8200.025.7854524
Produced by FastQC (version 0.11.2)