FastQCFastQC Report
Sun 14 Apr 2019
SRR6576291_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576291_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220061
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7160.32536433079918753No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3930.17858684637441438No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3740.1699528767023689No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3500.15904680974820617No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3140.14268770931696212No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2960.1345081591013401No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2900.1317816423627994No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2750.12496535051644772No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2740.12451093106002427No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2690.12223883377790704No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2630.11951231703936635No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2600.11814905867009602No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2520.11451370301870845No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2440.11087834736732088No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2430.11042392791089743No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2400.1090606695416271No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.10587973334666298No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2310.10497089443381608No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2260.10269879715169884No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2230.10133553878242851No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTACT250.005219276556.4304248
ATGGGTA1900.042.046635
TACATGG14850.034.184712
TGCTAAA550.002711132634.184712
CATGGGG6100.033.8968054
GTACATG15100.033.6646461
ACATGGG15200.033.0808073
TGGGTAC2150.032.7860226
GTATCAA8100.031.3787751
CCTACTA600.004142786431.3502379
CACCTAC600.00415664131.3288657
ATGGGGG2350.029.9957245
GGTACCT2250.029.260228
ATACTGG650.006147788428.9189556
TAGGACT650.006147788428.9189554
AACGCAG8500.028.7488426
AATACTG1154.180796E-628.6046165
TACCGTC1057.401987E-526.8533137
TTTGTAC700.008826924526.8533124
TTACACC700.008826924526.8533124
Produced by FastQC (version 0.11.2)