FastQCFastQC Report
Sun 14 Apr 2019
SRR6576292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187571
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.2505717834846538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.1284846804676629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA452.1938285E-762.6638264
CGCTAAA504.5443994E-756.397445
CCCGCTA504.5443994E-756.397443
GTATCAA6950.046.0080531
CTACGGA450.001012010241.7758839
ATGGGAT1209.640644E-1139.164895
GTACATG14350.037.6838871
GCAAACG500.001694330537.618351
GAATAAC757.3855936E-637.6183471
TATAGAC500.001698777137.5982973
TACATGG14550.037.146082
TTAGTCG456.17767E-736.55389838-39
ACATGGG14550.036.500063
TAGTACT651.4093619E-436.1522034
TCAACGC8750.035.986944
ATGCACT1055.0751623E-835.80794
CAACGCA8750.035.449825
AACGCAG9000.034.9873056
CATGGGA6900.034.7375564
TATCAAC9100.034.6028252
Produced by FastQC (version 0.11.2)