FastQCFastQC Report
Sun 14 Apr 2019
SRR6576292_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576292_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187571
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.35773120578341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.14980993863656963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.1236864973796589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC255.5489727E-575.1953053
TACTACG250.005230522256.396485
GTATCAA9250.048.958061
GTGTATA555.153047E-542.8846631
ATCAACG11250.039.6864133
CAACGCA11350.039.3367545
TCAACGC11300.039.0949064
TATCAAC11850.038.0837252
AACGCAG11800.037.8366246
CATGGGG6500.034.705534
GTCTTAG550.002662776534.307731
AAGACCG550.00271207234.1796845
ACGCAGA13300.033.5693367
GTACATG14750.033.2610551
CGCAGAG13700.032.5892078
TACATGG15200.032.473742
ACATGGG14700.031.6510853
GTCTATA600.004078098631.4487571
GTATAGT600.004078098631.4487571
AAGTGAC600.004153459331.3313777
Produced by FastQC (version 0.11.2)