FastQCFastQC Report
Sun 14 Apr 2019
SRR6576294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172775
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2510.14527564751844885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAAGC200.002162685470.4767763
GTATCAA3050.041.592851
GTACATG8050.034.4358561
ATCAACG3950.032.1159973
TACATGG8500.032.060022
ACATGGG8600.031.6872313
GTCCTAC600.00415799131.3230111
ATATAAC753.2704347E-431.3230111
GGTATCA1701.2187229E-1030.4017431
CAACGCA4200.030.204335
TCAACGC4200.030.204334
TCTGTAT1102.9568291E-629.8992373
AACGCAG4350.029.1628046
TTAACAC650.00614979228.9135483
CATGGGT2300.028.599274
TAAGGCT856.8056013E-427.6379494
TAGAGGT909.5031277E-426.1025094
GTACTTG909.5031277E-426.1025091
CCTCTAG909.5031277E-426.1025091
CGGAATC1101.01570986E-425.6279184
Produced by FastQC (version 0.11.2)