FastQCFastQC Report
Sun 14 Apr 2019
SRR6576294_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576294_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172775
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2550.14759079727969904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG250.00523248756.389234
GTATCAA3900.041.0140231
TATACTG608.80287E-539.1591875
ATCAACG4850.036.8177153
GTACATG8800.035.818731
TACATGG9000.035.5043332
ACATGGG9150.034.9222953
GACCGCG550.002713190234.1752937
TCAACGC5100.033.170144
CAACGCA5000.032.893725
CCCTTAA600.00413166831.3636651
GGTATCA1950.031.3636631
GTAGTGG753.2566936E-431.3454998
CATGGGA5100.031.3273534
AACGCAG5400.031.3273536
TATCAAC5850.030.5240882
CTTAATA953.738919E-529.6785453
ATGGGAG2550.029.4845685
AGGACCG650.006145630528.9175575
CGAGGTT650.006145630528.9175574
Produced by FastQC (version 0.11.2)