FastQCFastQC Report
Sun 14 Apr 2019
SRR6576295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377970
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5500.14551419424822076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7350.049.887871
ATCAACG9300.038.4063873
TCAACGC9250.038.105914
CAACGCA9350.037.698365
AACGCAG10250.034.388266
TACATGG17600.032.8533862
ACATGGG18000.032.1148153
GTACATG17850.031.8665351
TATCAAC12150.029.7921712
ACGCAGA12450.028.6891067
CATGGGA9750.027.4753384
CGCAGAG13000.027.113828
AACGGAT350.00883663126.85559366-67
CATGGGT5250.025.0652224
GGTATCA3200.024.9739171
GCAGAGT15100.023.3430239
AGAGTAC13450.022.53773110-11
ATGGGAG6050.022.5276285
GTTATAG1503.2853073E-521.9320683
GTGTAAT1303.149813E-421.6967961
Produced by FastQC (version 0.11.2)