FastQCFastQC Report
Sun 14 Apr 2019
SRR6576296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302130
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5470.18104789329096746No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3130.10359778903121172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA352.9387634E-453.696791
GTATCAA6900.044.2609371
TTAGCCC555.283965E-542.7133523
ACATGGG17300.038.0222973
TACATGG17500.037.3192672
GTACATG17550.037.2129441
CATGGGT5000.036.648064
ATCAACG8750.033.8289763
CAACGCA8950.033.5979925
CCTATAC702.1853842E-433.5604933
TCAACGC9100.033.0441784
GTATAAT2500.031.9495891
ATGGGTA3600.031.3231265
GGTACCT2700.031.3231268
ACCGGTG600.004162804731.3231267
ATACTGT1351.3245881E-831.3231266
AACGCAG9650.030.6739446
GGGTACC2950.030.2613247
CATGGGA9400.029.9902254
CGCGTAA404.7827154E-429.37029370-71
Produced by FastQC (version 0.11.2)