FastQCFastQC Report
Sun 14 Apr 2019
SRR6576296_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576296_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302130
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6230.2062026280078112No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3860.1277595736934432No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3620.1198159732565452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAA200.00215586470.545858
GTATCAA6000.056.4741061
TCAACGC7850.042.501994
CAACGCA8000.042.292485
ATCAACG8050.041.4460453
GTATAGG608.740731E-539.218131
AACGCAG8800.038.441346
GTACATG14950.037.4605261
TACATGG15250.036.05262
ACATGGG15450.035.281753
TATCAAC9850.033.8721472
CGTTATT702.183485E-433.5654562
TATAGGG702.183485E-433.5654562
ACGCAGA10300.032.3922967
CATGGGG5550.032.1744584
CGCAGAG10550.031.6509028
TATACGC2400.031.327767
TACGCTA2450.030.7138399
ATACGCT2450.030.7138398
ATGGGAG3600.030.0224385
Produced by FastQC (version 0.11.2)