FastQCFastQC Report
Sun 14 Apr 2019
SRR6576297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198231
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6220.31377534290802145No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4220.2128829496900081No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3810.1922000090803154No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3620.18261523172460412No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3130.15789659538619086No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2960.1493207419626597No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2930.14780735606438952No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2790.1407448885391286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.13267349708168752No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2600.13116011118341733No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2520.1271244154546968No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2450.12359318169206633No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2330.11753963809898553No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2310.1165307141668054No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2250.113503942370265No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2210.11148609450590474No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2210.11148609450590474No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC2210.11148609450590474No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2200.11098163253981466No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2100.105937012878914No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG2100.105937012878914No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2100.105937012878914No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2100.105937012878914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT200.002158216770.517411
CTTACCT250.005229778656.39974
ACCTAAG405.658395E-447.011611
TAACCTG450.001011950441.7775545
ATGGGGG1650.039.8785745
GTCTTGT500.001696571537.6092871
TAGACGC500.001698676337.59984
GTATCAA7650.037.4863781
ATGGGCA1650.037.03015
GTACATG15450.036.513871
TACACAG651.4093192E-436.1536525
ACATGGG15700.035.6240123
CATGGGT3850.035.4024054
TACATGG16150.034.9312252
TCTAGAC550.002711683334.1816373
TAGTACT550.002711683334.1816374
CATGGGG4150.033.9757234
CCTAAGA702.1753514E-433.579722
AGGTCGG300.004157833331.33316686-87
ATGGGTA3000.031.3331645
Produced by FastQC (version 0.11.2)