FastQCFastQC Report
Sun 14 Apr 2019
SRR6576297_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576297_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198231
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6970.35160999036477647No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5070.2557622168076638No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4490.22650342277443994No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4110.20733386806301737No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3990.2012803244699366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.18715538941941473No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3550.17908399796197363No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3190.16092336718273126No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3010.15184305179311006No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2970.1498252039287498No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2840.14326719836957894No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2830.14276273640348885No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2630.13267349708168752No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2470.12460210562424646No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2460.12409764365815638No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG2320.11703517613289545No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG2310.1165307141668054No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2260.11400840433635506No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2260.11400840433635506No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2240.11299948040417493No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2160.1089637846754544No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC2150.10845932270936431No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2140.10795486074327426No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC2130.10745039877718418No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG2120.10694593681109413No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2030.10240577911628353No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2030.10240577911628353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG405.670614E-446.9910475
GTATCAA8700.043.8942381
GGGTACC2900.043.7502827
TGGGTAC3150.040.2780346
GGTACCT3050.040.0579388
GACATAT608.638648E-539.2880481
CATGGGT4200.039.1592034
GTTACAG608.807555E-539.1592033
ATGGGTA3500.037.592845
ACTCCGG500.001700222437.5928348
GTACATG16350.037.1974951
TACATGG16750.036.470662
ACATGGG16600.036.234063
TGTATCG651.410855E-436.1469574
GTACCTG3500.034.9076359
CAACGCA11150.034.5584375
ATCAACG11100.034.2907643
CATATAG550.002714146934.1753043
GAGCCGA550.002714146934.1753049
TCAACGC11200.033.9845964
Produced by FastQC (version 0.11.2)