FastQCFastQC Report
Sun 14 Apr 2019
SRR6576299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463454
Sequences flagged as poor quality0
Sequence length100
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11010.2375640300871284No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6610.14262472651007435No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5200.1122009951365184No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5160.1113379105585452No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5160.1113379105585452No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4910.10594363194621258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10000.044.6615371
GTACATG29000.035.664381
ACATGGG28150.035.2268263
TACATGG29350.034.7585532
CATGGGG14350.033.405484
TCAACGC13950.031.6681794
GTATTGA1052.057699E-631.3414271
ATCAACG14450.030.5723953
CAACGCA14050.030.1047925
CACGACC650.00615239728.9211864
TATGCGC650.00615239728.92118694
CATGGGT7500.028.8247814
ATGGGTA5400.028.7203435
AACGCAG14600.028.6488116
TATCAAC16500.027.6374422
ATGGGGA7450.025.2332465
ATGGGGG5600.025.1769245
GGTACCT5900.024.6932988
ATGGGCG1351.4557409E-524.3687745
ACGCAGA17300.024.177617
Produced by FastQC (version 0.11.2)