FastQCFastQC Report
Sun 14 Apr 2019
SRR6576299_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576299_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463454
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13610.2936645276553876No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7830.1689488061382575No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7080.15276597030125966No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6990.15082403000081993No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5630.12147915434973051No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5370.11586910459290459No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5330.11500602001493136No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5300.11435870658145146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.1074540299576657No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4700.10141243791185317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG250.00523569156.39562
GTATCAA9900.049.508761
ACATGGG22450.037.8901373
GTACATG23850.037.3471371
TACATGG23600.037.238622
TCAACGC13450.035.9897584
CATGGGG9750.035.669014
ATCAACG13600.035.5928083
CAACGCA13750.034.8627325
AACGCAG14050.034.1183366
GTATAGA753.220546E-431.419021
TCTATAC753.274047E-431.3308893
CATGGGT6200.031.078224
ACGCAGA15800.030.3394057
TTATACC804.7787154E-429.3727074
TATCAAC16750.029.1798132
ATGGGTA4350.029.1701375
GGATACG650.006068901629.002171
CGCAGAG17000.028.19788
GTATATA1554.937101E-827.3649541