FastQCFastQC Report
Sun 14 Apr 2019
SRR6576300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407616
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11440.2806563039723662No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6490.15921848013816925No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5230.12830703407128277No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5010.12290979745642958No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4900.12021117914900298No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4510.1106433506044905No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4270.10475545611555975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC1007.057679E-1042.291944
GTATCAA10650.037.945821
ACATGGG25550.034.7604983
ATGGGTA4350.034.5681275
GTACATG25700.034.191931
TACATGG26200.033.7187652
CATGGGT7100.032.430444
ATCAACG12800.030.4707533
TCAACGC12950.030.1178134
GTCTTAT1252.3607936E-730.0742681
CATAATA1452.6560883E-829.1668552
CAACGCA13250.029.0812515
AACGCAG13300.028.9719226
GGTACCT5050.028.8424518
GGGTACC5150.028.2824027
CATGGGC5300.027.4853274
CTTATAG1205.8397054E-627.4114423
TGGGTAC5400.026.976346
ATAATAC1057.4196425E-526.8520263
GTTAAGC909.5176825E-426.1061363
Produced by FastQC (version 0.11.2)