FastQCFastQC Report
Sun 14 Apr 2019
SRR6576300_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576300_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407616
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13380.3282501177578898No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8010.19650847856806405No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7000.1717302559271471No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6260.15357591458627728No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5700.13983749411210553No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5340.13100565237870937No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5150.12634440257497254No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4570.11211532422672318No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4550.11162466635264562No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4460.10941670591929659No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.10671808761187No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4300.10549144292667609No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG4290.1052461139896373No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4280.10500078505259854No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4220.10352881143036584No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4130.10132085099701679No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC405.6763977E-446.993253
GTATCAA8900.042.858541
ACCGGTC450.001010232541.8025558
GTACATG24050.038.9655081
TACATGG24650.037.937762
ACATGGG24100.037.6336023
ATACTGC801.1579559E-535.2449386
CATGGGT7150.032.2051664
CCATATA753.2347554E-431.3943181
AAACGTA300.004165703431.3249974-75
TCAACGC11800.031.0633374
AACGCAG12400.030.6972066
CATGGGG11100.030.482114
ATCAACG12200.030.430063
CAACGCA12150.030.1685075
CTTACAC1252.3598295E-730.0756843
CATGGGA8650.029.88014
CATGGGC4200.029.0910634
TTTAGAG650.006153885328.9189243
GTCTAGA1554.9718437E-827.343441
Produced by FastQC (version 0.11.2)