FastQCFastQC Report
Sun 14 Apr 2019
SRR6576301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279902
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3770.13468999864238196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6500.039.044671
TACATGG13000.037.237052
GTACATG13250.036.8891641
ATAACGC550.002714020734.180523
ACATGGG14350.033.4063953
CATGGGT3550.031.7734414
ATCAACG7950.031.3321423
GTACTAG753.2699926E-431.332141
TCAACGC8150.031.1399214
CAACGCA8500.030.410615
GTATAAG1102.958761E-629.9079531
GGGTACC2101.8189894E-1229.0941317
AACGCAG8900.029.043846
CTAGTAC650.00614746728.9219783
ACGCAGA9000.028.7211327
CATGGGA7950.028.376284
ATGGGTA2350.027.9989365
CGCAGAG9500.027.7042088
GTATAGC856.804633E-427.6460081
TATCAAC9250.027.4367942
Produced by FastQC (version 0.11.2)