FastQCFastQC Report
Sun 14 Apr 2019
SRR6576302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249133
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3780.15172618641448543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC301.3696561E-462.6611821
GTATCAA7150.032.8642581
GTCTTAT600.004156564431.3305911
GTACATG14000.031.2186931
TACATGG13950.030.99372
ACATGGG14550.030.0325783
AACGCAG8200.028.0772696
TAGACAC856.8124774E-427.6390885
CATGGGT4100.027.5042654
TGAGCGG700.00883539626.8494015
ATCAACG8400.026.2900373
GTAATAT909.501545E-426.1088241
CAACGCA8600.025.6786425
ATGGGTA2750.025.6289735
TGGGTAC1859.988071E-925.3980836
TCAACGC8700.025.3834864
CTAAGGG1301.0858004E-525.3003984
GTGTTAG1501.1573084E-625.0644721
CCTCTAT950.001302383424.7346761
CTTATAT950.001303908524.7297133
Produced by FastQC (version 0.11.2)