FastQCFastQC Report
Sun 14 Apr 2019
SRR6576302_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576302_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249133
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4810.19306956525229496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3650.1465080900563153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.11720647204505223No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2780.11158698365933055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8100.041.287831
AACGCAG8550.038.478716
GAGCGAC250.00169792737.614366-67
ATCAACG9050.036.345523
TCAACGC9100.035.6294484
CAACGCA9250.035.0516745
CGCAGAG9900.033.7266658
GTCTATA702.1522428E-433.6450161
ACGCAGA9900.033.2316137
GTACATG13950.033.090291
TACATGG14100.032.9926722
CATGGGT3150.032.8181084
CATGGGG5950.032.3793644
ACATGGG14700.031.6460293
GCCCTAA600.004110482531.4020121
TATCAAC10900.031.0389752
ATGGGTA2300.030.6453675
ACTATAT650.006151715328.9166533
GTGCAAC1005.228397E-528.261811
GTACTTG1503.59596E-828.2618081
Produced by FastQC (version 0.11.2)