FastQCFastQC Report
Sun 14 Apr 2019
SRR6576303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508517
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.13942503397133232No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5200.10225813492960904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13100.042.376661
CAACGCA16950.032.44185
ATCAACG16950.031.887243
TCAACGC16950.031.887244
TACATGG26200.031.7606122
AACGCAG17800.031.420696
GTACATG26450.030.7706321
ACATGGG26950.029.4724833
TATCAAC18900.028.854472
ACGCAGA19850.027.702197
CATGGGT6150.026.7474064
CGCAGAG20600.026.6936178
ATGGGTA3400.024.881835
CATGGGG11650.024.6089274
GTCTTAC1751.6271952E-724.1947141
GCAGAGT23800.022.9070859
CTTAGAC1652.6594716E-622.7874033
GGTATCA6600.022.0970251
GTACTTG3650.021.9114971
ATGGGAG5850.020.8884535
Produced by FastQC (version 0.11.2)