FastQCFastQC Report
Sun 14 Apr 2019
SRR6576303_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576303_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508517
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.16695607029853476No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5160.10147153389168898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10900.046.7197881
CCTATAC1152.7721399E-936.7784923
CAACGCA14350.034.7138865
ATCAACG14550.034.2367173
TCAACGC14700.034.2070544
AACGCAG14800.033.9759256
TACATGG24800.033.92962
ACATGGG24700.033.4861343
GTACATG24650.033.475321
TATCAAC16150.030.8539452
GGTATCA5450.029.4161621
CATGGGG11050.029.3451334
GGTACCT2750.029.0512948
ACGCAGA17800.028.2496477
CGCAGAG18850.026.925378
AACGCCG1101.0180168E-425.6334935
ATGGGTA3500.025.511435
ATGGGGA6900.025.2000775
GGGTACC3400.024.8795687
ACGCCGC1151.3767794E-424.5189956
Produced by FastQC (version 0.11.2)