FastQCFastQC Report
Sun 14 Apr 2019
SRR6576306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361497
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6060.16763624594394974No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3690.10207553589656344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG250.005230340656.407642
GTATCAA9950.035.431931
GTACATG22050.034.961651
TACATGG22650.033.6204452
ACATGGG22100.033.1763763
ATCAACG12000.029.3749123
AACGCAG12000.028.9832486
CATGGGG8000.028.7874154
TCAACGC12250.028.7754274
CAACGCA12200.028.5081145
CATGGGT5050.026.990024
GTAATAG1057.400922E-526.8607791
CTATTAA1057.400922E-526.8607791
TATCAAC13900.026.7158492
CATGGGA11450.026.2706644
ATGGGAG5550.025.405335
CGCAGAG13750.025.2944728
ACGCAGA14150.024.5794337
CATGGGC4050.023.2098084
TTATACC1100.003055586521.3635734
Produced by FastQC (version 0.11.2)