FastQCFastQC Report
Sun 14 Apr 2019
SRR6576306_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576306_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361497
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6070.1679128734125041No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.10843796767331404No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3760.10401192817644407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10050.046.920291
AACGCAG12200.038.9084666
TCAACGC12550.036.6998984
ATCAACG12600.036.5542643
CAACGCA12650.036.0382545
GTACATG20350.033.5993081
TATCAAC14550.032.947292
TACATGG20700.032.9215432
ACGCAGA14450.032.5248037
ACATGGG20400.032.4841463
CGCAGAG14550.031.9782528
GTATAAA1859.094947E-1230.5869561
GCAGAGT16350.027.8828079
AGAACCG1057.409528E-526.8561955
CCATAAC1101.01668906E-425.635463
CATGGGA11400.025.56054
CATGGGG9250.024.8964184
AGGGCGT950.001303124724.7359688
AGAGTAC15300.024.57429710-11
GTACTTT9550.023.700891
Produced by FastQC (version 0.11.2)