FastQCFastQC Report
Sun 14 Apr 2019
SRR6576307_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576307_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326657
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6420.19653642811879127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.13010589088860794No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3540.10837055382251107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10150.040.805391
AACGCAG10950.038.1954426
TACATGG21100.036.9710042
GTACATG21050.036.444871
ACATGGG21200.036.1316153
TCAACGC11550.035.804394
ATCAACG11600.035.6500633
CAACGCA11650.035.4970555
ACGCAGA12800.032.6750037
CGCAGAG13050.032.0686848
CATGGGG8700.030.2485374
TATCAAC14050.030.1024482
CATGGGC4000.029.3707894
TCTATAC804.7774875E-429.3707893
CATGGGA10200.028.5645334
GCAGAGT14950.027.9930639
ATGGGAC2104.9112714E-1126.8532945
ATAAGAC700.00883310426.8532923
TAGGGGC700.00883310426.8532924
TAGGCAT700.00883310426.8532925
Produced by FastQC (version 0.11.2)