FastQCFastQC Report
Sun 14 Apr 2019
SRR6576308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234539
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6650.28353493448850725No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3720.1586090159845484No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3690.15732991101693108No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3210.13686423153505387No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3020.1287632334068108No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2880.12279407689126329No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2810.11980949863348953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.11042939553762915No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2580.11000302721509002No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2530.10787118560239449No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2520.10744481727985536No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2370.10104929244176876No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2360.10062292411922964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC405.6685036E-446.9981883
GTATCAA6250.045.870231
GTACTAG450.001012495741.776171
GTATAGA450.001012495741.776171
GTACATG14250.037.928361
ACATGGG14600.037.662933
GTATAAC500.001699588437.5985531
CTCTATA757.4202762E-637.5985532
CATGGGG6800.036.6309434
TACATGG14850.036.39592
TCAACGC7950.035.470334
ATCAACG8000.035.2486423
AACGCAG8350.034.896866
CATGGGT4200.034.6891374
TATACAG959.410942E-734.630245
CAACGCA8500.033.728115
TATATAC702.180218E-433.5701333
ATAACAC851.7493205E-533.1751943
ACGCAGA8950.032.5574047
ATGGGTA2550.031.3321255
Produced by FastQC (version 0.11.2)