FastQCFastQC Report
Sun 14 Apr 2019
SRR6576309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220388
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6720.304916783127938No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3850.17469190700038115No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3750.17015445487050113No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3520.15971831497177705No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3010.1365773091093889No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2850.12931738570158086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.12704865963664083No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2780.12614116921066482No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2710.1229649527197488No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2590.11752001016389277No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2480.11252881282102474No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2360.1070838702651687No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2320.10526888941321669No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2320.10526888941321669No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2270.10300016334827666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT451.6122376E-552.2081031
GTAACAC601.6021731E-646.9872933
GTATCAA7050.045.3210751
AACGCAG8450.036.144076
TCAACGC9100.034.595044
ATCAACG9150.034.4059943
CATGGGG5350.034.252424
CAACGCA9100.034.0786935
TAGAGGG702.1827614E-433.562354
GTACATG14100.031.9913481
TACATGG14150.031.8783052
ACATGGG13600.031.785523
GGATAGA600.004159254531.3248626
TCCTATA600.004159254531.3248622
ATGGGGA2900.030.784785
ACGCAGA9950.030.6952177
CGCAGAG10000.030.5417398
TATCAAC10850.029.4482572
TCGCTCA650.006151646428.9152579
GCAGAGT11100.027.515089
Produced by FastQC (version 0.11.2)