FastQCFastQC Report
Sun 14 Apr 2019
SRR6576310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309448
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6390.20649672966055688No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3910.12635402393940176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.10890359608076317No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3180.10276363072309402No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3150.10179416250872522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA254.9088703E-794.012123
CCGCTAA353.6329875E-667.140664
CGCTAAA451.6114984E-552.2205165
GTATCAA8400.047.0288661
TACATGG21550.039.063422
GTACATG22100.038.091251
ACATGGG22250.037.8161133
GACCGTC959.4234383E-734.6304477
CAACGCA11700.034.1441845
AACGCAG11900.033.570336
CATGGGA11100.033.0259484
CATGGGG7300.032.8345454
ATCAACG11750.032.804233
TCAACGC12100.032.6270334
GTATAGA1052.0490788E-631.3525771
ATGGGTA2700.031.332315
CATGGGT5850.031.332314
TATCAAC13250.029.8145262
GCTATGC650.00612889828.940841
ACGCAGA13600.028.6828867
Produced by FastQC (version 0.11.2)