FastQCFastQC Report
Sun 14 Apr 2019
SRR6576310_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576310_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309448
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5760.18613789715881182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.12506139965357668No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3730.1205372146531889No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3210.10373309893746284No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3190.1030867867945503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG20050.037.973892
GTACATG20600.037.2966581
ACATGGG20600.036.9600223
GTATAGG1152.6848284E-936.8887751
GTATCAA9200.036.376431
CATGGGG6200.034.869894
CATGGGT5400.034.813744
ATGGGGC2200.032.0444645
AACGCAG11050.031.4741386
TTGGCGT300.004160140631.33236550-51
ATGGGAG5450.031.0449125
ATCAACG11000.030.7626843
TCAACGC11150.030.3488374
ATGGGTA3350.029.4617735
TACCATA804.7737683E-429.374097
CAACGCA11650.029.0463125
CATGGGA11450.028.3222684
ACGCAGA12250.028.0072967
ATGGGTG2700.027.850995
CGCAGAG12350.027.3999618
Produced by FastQC (version 0.11.2)