FastQCFastQC Report
Sun 14 Apr 2019
SRR6576311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280650
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5640.20096205237840728No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3630.1293425975414217No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.11437733832175306No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3110.11081418136468912No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2900.1033315517548548No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2880.10261892036344201No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2880.10261892036344201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCA250.005238212656.3828969
CAGTACT1551.8189894E-1236.3760644
ACATGGG19650.033.9540753
GTACATG20250.033.894261
TACATGG19850.033.8667762
GTATAAG1001.4035904E-632.907611
CATGGGG6900.031.3238324
GTATCAA10250.031.18771
GTATTGG953.7363796E-529.6910781
CACCTCG953.7500424E-529.6752097
CATGGGT4900.028.7667854
CATGGGA10450.027.4270884
AACGCAG11550.027.2558026
CCAGTAC1406.3563493E-726.8489993
ATGCACC1755.5333658E-926.8489994
TAAGACT1406.3563493E-726.8489994
ATGGGAT2208.731149E-1125.628595
ATCAACG12300.025.593863
TCAACGC12450.025.2855014
ATGGGTA2800.025.1709375
Produced by FastQC (version 0.11.2)