FastQCFastQC Report
Sun 14 Apr 2019
SRR6576311_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576311_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280650
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5840.20808836629253516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.14359522536967753No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3570.1272047033671833No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3220.11473365401745945No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3200.11402102262604667No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3050.10867628719045073No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3000.10689470871191875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9050.041.6046141
ACATGGG18150.033.397683
GTACATG18900.033.368991
TACATGG18800.033.2486762
ATCAACG11300.032.4354063
AACGCAG11350.032.292526
TCAACGC11500.031.8713134
CAACGCA11750.031.19325
CATGGGG6050.030.2909164
GGGTACC2051.8189894E-1229.7983827
TATCAAC12900.029.1461282
ACGCAGA13150.027.872257
CGCAGAG13250.027.681628
TATATGG856.804748E-427.6459582
ATGGGGA3400.027.6410335
ATGGGTA2600.027.1094725
CGTAAAA350.00880328726.87522970-71
TCGTGGG700.00880357126.8704389
CGTAGAG700.00882656326.8560732
GTCGTTC350.00883395326.85607382-83
Produced by FastQC (version 0.11.2)