FastQCFastQC Report
Sun 14 Apr 2019
SRR6576312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302662
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6310.20848339071307267No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3930.12984781703682655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.10341569143136567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA156.8980776E-493.997193
CGCTAAA250.005232943756.398315
GTATAAC704.624868E-640.284511
GTACATG16650.038.1069681
TACATGG16750.037.3182872
ACATGGG17150.036.4478873
GTATCAA7200.034.596191
GTGCTAT702.1815985E-433.5704231
GTAAGAC600.00415679131.3323973
CATGGGT4700.030.9990734
AACGCAG8700.030.7921856
CATGGGA9000.029.243574
TCCTGCG650.006148002628.922212
TATAACG650.006148002628.922212
ATCAACG9000.028.1991583
TCAACGC9150.027.7368774
CAACGCA9650.027.2737985
ATGGGAT2600.027.1145735
GAGTAAG700.00882721226.856341
CATAGAC700.00882721226.856343
Produced by FastQC (version 0.11.2)