FastQCFastQC Report
Sun 14 Apr 2019
SRR6576312_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576312_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302662
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5330.1761040368463831No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.11531014795382309No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3440.11365814010348178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG18800.034.8405081
TACATGG19050.034.5505182
ATGGGAG4250.034.2921185
TACGCTC550.002714469634.18025694
GTATCAA10950.033.996991
ACATGGG19100.033.4755023
CCTACAC1457.366907E-1032.4230233
GTACTAA600.004103593531.4149421
CATGGGG5600.031.0624164
AACGCAG12350.030.073346
GTATTAT1102.8995219E-629.986991
TCAACGC12450.029.454174
GCTCTAA804.7004974E-429.4515081
CAACGCA12800.029.3833665
CTAATGG650.00613855728.9313133
GACTCGT650.006148478528.9217537
ATCAACG12900.028.4266993
CATGGGA10600.028.3854394
CGCAGAG14000.027.1916168
ACGCAGA13900.027.0491247
Produced by FastQC (version 0.11.2)