FastQCFastQC Report
Sun 14 Apr 2019
SRR6576314_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576314_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337521
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9400.2785011895556128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.1632491015373858No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5380.15939748934140394No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5200.15406448783927518No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4740.14043570622272392No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4590.1359915383042833No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4100.1214739231040439No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4000.11851114449175015No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3940.11673347732437389No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3930.11643719946314451No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3560.10547491859765763No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3540.10488236287519888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG352.8923512E-453.871961
GTATCAA8050.051.5296971
TGTATAC952.1100277E-839.5775153
ATCAACG10650.038.8342743
TCAACGC10600.038.5740744
CAACGCA10550.038.311415
AACGCAG11000.037.171386
GTATACT1204.1891326E-935.2487224
GTACATG16100.033.0844081
TATCAAC12800.032.6930352
ACCTAAG1457.166818E-1032.508941
ACATGGG16250.032.3926733
TACATGG16500.031.916062
CATATAT902.537188E-531.425311
ATTAGCC600.00415759431.3321953
GGGTACC3500.030.8845947
TGGGTAC3600.030.026696
CATGGGT4900.029.7336164
ATGGGTA3800.029.6831365
ACGCAGA13950.029.3107667
Produced by FastQC (version 0.11.2)