FastQCFastQC Report
Sun 14 Apr 2019
SRR6576319_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576319_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774151
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17610.2274750016469655No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13770.1778722755638112No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11290.14583718163510737No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10240.13227393622174485No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG9630.12439433650541046No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT9280.11987325470095628No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG9100.11754812691580842No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA8790.11354374017472042No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG8610.11121861238957258No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC8330.10760174694600924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.10359736020492127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17550.046.9611931
ACATGGG42500.037.158493
GTACATG43950.036.111811
AACGCAG23300.035.704676
ATCAACG23200.035.453393
TCAACGC23000.035.3529744
TACATGG45550.034.876752
CAACGCA23650.034.77885
TGCGTAT753.274125E-431.33404594
CATGGGG21300.030.8927214
ACGCAGA27850.030.3757467
CATGGGA17100.030.2346024
CGCAGAG29250.028.9218658
GTATAGG1802.3283064E-1028.78051
TATCAAC29550.027.834812
ATGGGTA8300.027.747625
CATGGGT12450.026.803824
GCAGAGT32200.026.2721929
ATGGGGA12400.025.3957395
ATGGGAG10100.025.129285