FastQCFastQC Report
Sun 14 Apr 2019
SRR6576321_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576321_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277023
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5080.1833782754500529No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3560.12850918515791107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.12092858715702305No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3240.11695779772798649No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3190.11515289344206077No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3170.11443093172769049No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3150.11370897001332018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG17750.038.92942
GTACATG18200.038.3289381
ACATGGG18600.037.1503683
CATGGGT3100.036.39224
GTATCAA9550.034.05511
CATATAC902.5843985E-531.3377273
CTATACT902.5843985E-531.3377274
TCTATAC600.00415273131.3377273
ACTATAC753.26995E-431.332078
CATGGGG7500.030.7109724
AACGCAG11150.029.5108646
ATCAACG11200.028.5397153
GTATTAA1005.2069194E-528.2805391
TCAACGC11550.028.081864
GCGTTAT1354.499634E-727.9313981
CAACGCA11700.027.7218345
ATAGTAC700.00881862426.8609093
TGCGACG350.00883387926.8560622-23
GAGGCGC700.00882639326.856069
CACTCTA1807.4487616E-926.110069
Produced by FastQC (version 0.11.2)