FastQCFastQC Report
Sun 14 Apr 2019
SRR6576322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252845
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5680.22464355632897626No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3480.13763372817338684No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3240.12814174692004984No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3170.12537325238782654No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3040.12023176254226899No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2920.11548577191560047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.11311277660226622No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2620.10362079534892918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG853.9475526E-738.695625
ACGACGG959.4314964E-734.62246
GTATAAG550.002716549234.1727561
TACATGG17700.033.979692
GTACATG17900.033.600031
ACATGGG17850.032.9044383
CGACGGT1001.4083143E-632.8912777
GACGGTA1052.0612115E-631.3250278
GTCTTAT753.2734603E-431.3250271
ACGGTAT1052.0612115E-631.3250279
CATGGGG6500.029.6382944
GTATAGT804.7778725E-429.3672141
ATGGGAG3350.028.0522615
CATGGGC3400.027.6397294
GTATCAA9400.027.4927081
CTACGAC1205.831589E-627.4093974
ATGGGGG2450.026.8500235
TAATACT1057.412451E-526.8500234
GTAATAG700.00883464126.8500211
ACATAGG700.00883464126.8500213