FastQCFastQC Report
Sun 14 Apr 2019
SRR6576323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364909
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8040.2203289039185112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.12441458007338817No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4290.11756355694159366No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4200.11509718861414764No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4120.11290486121197339No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3970.10879424733289669No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3830.10495767437909177No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3730.10221726512637398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG156.8987673E-493.9987645
ATATCGC156.8987673E-493.9987646
TACACGA405.671964E-446.9993865
GTATTAT704.6226014E-640.290711
GGGCGCA500.00170061537.5995067
TAGTGCC500.00170061537.5995067
GTATCAA12100.036.90541
GTACATG25300.036.787171
CATGGGT5500.035.8904384
TACATGG26050.035.7280352
ACATGGG26400.035.249543
GGTACCT3950.034.5058758
ATGGGTA4150.033.9754565
CATGGGC5000.031.9595814
GGGTACC4150.031.7104267
GTCTAGC902.5869209E-531.3372141
ACCGCTT600.004157562331.332928
AACGCAG15200.029.3746176
ATGGGCG650.006149135528.9226975
TGGGTAC4400.028.840536